P-88: Mast Cells Number and Distribution and Myoid Cells Population Are Impressed by Sulpiride Antipsychotic Drug

Background: Spermatogenesis is under the control of variety of factors, which include mast cells. Any changes in testicular mast cells’ number and distribution resulting in deterioration of comlex regulation and fine-turning of spermatogenesis. Increased number of mast cells and their abnormal spread pattern have been described in the testis with fertility disturbances. Mast cells’ tryptase enzyme is a potent mitogen for myoid cells and can enhance the chemotaxis and activation resulting in seminiferous tubular fibrosis. Also these cells may be involved in testicular inflammatory disorders. In present study the effect of sulpiride antipsychotic drug on testicular mast cells number and distribution and, consequently, myoid cells population changes in mature male mice were investigated. Materials and Methods: 18 adult male mice were categorized in to 3 groups. Treatment group was administered sulpiride 40 mg/kg/day IP for 45 days. Control sham received sesame oil as a vehicle of drug and control group received nothing. 45 days later testicular tissue samples were taken for histological studies using Toluidine-blue (mast cells) and Iron-weigret (myoid cells) staining methods. Results: Increased number of mast cells and their wide distribution around seminiferous tubules and in interstitial tissue and, in some cases, in tubular lumens were observed in test group in comparison with controlsham and control groups. Increase in the interstitial fluid showed inflammation in test group samples. Additionally, myoid cells populated significantly in test group in compare with 2 other groups (p<0.05). Conclusion: Mast cells high and wide distribution because of sulpiride atypical antipsychotic drug administration, resulting in proliferation and activation of myoid cells and edema induction, cause pathological changes in testicular structure that increase the risk of fertility problems in some cases.